Harlequin mLGA

Product Code: HAL009

A selective chromogenic media for the simultaneous enumeration of Escherichia coli and coliforms.

Application(s): Industrial, Water
Organism(s): Enterobacteriaceae / Coliforms, E.coli

Traditionally, membrane Lauryl Sulphate Broth (mLSB) has been used as the standard media for isolating coliforms (including E. coli) from water potentially contaminated with sewage. Harlequin^TM membrane Lactose Glucuronide Agar (mLGA) is a modification of mLSB aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and E. coli. The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide, sodium pyruvate and agar. X-glucuronide is incorporated to allow for the presumptive isolation of E. coli, sodium pyruvate aids the recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads.

This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Microbiology of Drinking Water 2002 (previously Report 71).